SAXS and the Working Protein

pyDockSAXS: protein–protein complex structure by SAXS and computational docking

Structural characterization of protein-protein interactions at molecular level is essential to understand biological processes and identify new therapeutic opportunities. However, atomic resolution structural techniques cannot keep pace with current advances in interactomics. Low-resolution structural techniques, such as small-angle X-ray scattering (SAXS), can be applied at larger scale, but t...

Reconstruction of SAXS Profiles from Protein Structures

Small angle X-ray scattering (SAXS) is used for low resolution structural characterization of proteins often in combination with other experimental techniques. After briefly reviewing the theory of SAXS we discuss computational methods based on 1) the Debye equation and 2) Spherical Harmonics to compute intensity profiles from a particular macromolecular structure. Further, we review how these ...

: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes

هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...

Nanostructure of hydrated wheat protein gliadin as revealed by SAXS

Structural analysis of the intrinsically disordered choloroplast protein CP12, using NMR and SAXS

In order to decipher the mechanistic details of the metabolic regulation of the green alga Chlamydomonas reinhardtii, we are working on the molecular characterization of the regulation of Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and Phosphoribulokinase (PRK), two key enzymes of the Calvin cycle, by the chloroplast protein CP12. A careful analysis of the sequence of CP12 reveals that the...